PCR detection of host and HIV-1 sequences from archival brain tissue.

Detection of single Dactylogyrus spp. in DNA extracted from infected gill tissue of fishes using Polymerase Chain Reaction

Dactylogyrus spp. are monogenean worms found mostly as ectoparasites on the gills of several fish species, including carp and goldfish. These parasites are commonly detected by microscopic analysis of the gill lamellae, but this is time-consuming and technically difficult. In contrast to this conventional method, molecular techniques provide specific, sensitive and safe detection of parasites. ...

Detection of Ornithobilharzia turkestanikum cercaria (trematoda) by nested-PCR in intermediate host snail, Lymnaea gedrosiana

  Trematodes are important in economic and public health. Ornithobilharzia turkestanicum (O. turkestanicum) is one of the important economic trematodes in domestic animals. Ornithobilharzia infection in intermediate host (Lymnaea gedrosiana ) can be detected by either exposing snails to light to induce cercarial shedding or by squeezing them between glass slides to detect parasites. The current...

Detection of NAD(P)H: Quinone Oxidoreductase 609C T Polymorphism in Blood and Archival Human Tissues Using a Simple PCR Method

Detection of herpesvirus-like DNA by nested PCR on archival skin biopsy specimens of various forms of Kaposi sarcoma.

AIMS To detect herpesvirus-like DNA sequences, defining a new herpesvirus, human herpesvirus 8 (HHV8), in paraffin wax embedded skin biopsy specimens of the various forms of Kaposi sarcoma. METHODS DNA was extracted from archival skin biopsy specimens of Kaposi sarcoma, other mesenchymal skin tumours and various inflammatory skin lesions of HIV seropositive and negative patients. HHV8 DNA was...

Development of 16S rRNA targeted PCR methods for the detection of Escherichia coli in Rainbow trout (Oncorhynchus mykiss)

  Objectives: The presence of E.coli in fish intended for human consumption may constitute a potential danger, not only in causing disease, but also because of the possible transfer of antibiotic resistance from aquatic bacteria to those infecting humans. The objective of this study was to develop an improved PCR method based on species – specific 16 S rRNA gene primers (FES,...